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Vcantm1.1Dzim
Targeted Allele Detail
Nomenclature
Symbol: Vcantm1.1Dzim
Name: versican; targeted mutation 1.1, Dieter R Zimmermann
MGI ID: MGI:3850559
Synonyms: VCAN(tm1Zim), VersicanV0/V2-
Gene: Vcan  Location: Chr13:89655312-89742509 bp, - strand  Genetic Position: Chr13, 45.5 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:150124
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsAn endoplasmic reticulum retention signal (KDEL) followed by a STOP codon and floxed neo cassette was inserted into exon 7 after codon E749. This exon encodes the GAG-alpha domain. Germ line, cre mediated recombination removed the neo cassette. Quantitative RT-PCR on brain extracts confirmed a 90% reduction in isoform V2 transcript expression and a decline of isoform V0 transcript to 20% (at P0) or 50% (at P30 and P100) of the levels in wild-type mice. Transcript expression of isoform V1 and V3 is unaffected. Immunohistochemistry confirmed the absence of protein expression of isoforms V0 and V2. (J:150124)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Vcan Mutation:  9 strains or lines available
References
Original:  J:150124 Dours-Zimmermann MT, et al., Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. J Neurosci. 2009 Jun 17;29(24):7731-42
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory