Kmt2a^tm1Brad
Targeted Allele Detail

Summary

• Symbol: Kmt2a^tm1Brad
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1, Hugh JM Brady
• MGI ID: MGI:3849322
• Synonyms: Mll^flox
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^tm1Brad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149811
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was constructed where an upstream loxP site was cloned into a blunted NsiI site in intron 7 and a floxed neomycin resistance cassette was cloned into a BglII site in intron 9. Targeting took place in PC3 ES cells homozygous for the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase in male germ cells. Cre mediated recombination subsequently removed only the neomycin cassette upon transmission from male chimeric mice. Offspring from the male chimeras in which exons 8 and 9 remained floxed were selected for further breeding. Expression was unaffected as determined by immunoblot analysis of splenic extracts. (J:149811)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 135 strains or lines available

References

• Original: J:149811 McMahon KA, et al., Mll has a critical role in fetal and adult hematopoietic stem cell self-renewal. Cell Stem Cell. 2007 Sep 13;1(3):338-45
• All: 5 reference(s)