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Dlg4tm2.1Grnt
Targeted Allele Detail
Nomenclature
Symbol: Dlg4tm2.1Grnt
Name: discs, large homolog 4 (Drosophila); targeted mutation 2.1, Seth G N Grant
MGI ID: MGI:3848928
Synonyms: PSD-95GK
Gene: Dlg4  Location: Chr11:70017085-70045532 bp, + strand  Genetic Position: Chr11, 42.96 cM, cytoband B4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:89778
Parent Cell Line:  E14TG2aIV (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA STOP codon and coding sequence for haemaglutinin (HA) epitope tag were inserted in frame before the start of the GK domain at amino acid 500. In addition, a floxed neo cassette was inserted downstream to replace sequence and this cassette was subsequently excised by Cre leaving a single loxP site after the STOP codon. Western blot and immunoprecipitation analysis confirmed the absence of protein in hippocampal extracts from homozygous mice. RT-PCR analysis indicated that a very low level of mRNA expression (less than 5% of wild-type) was present in hippocampal extracts from homozygous mice. (J:149508)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dlg4 Mutation:  6 strains or lines available
References
Original:  J:89778 Yao WD, et al., Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticity. Neuron. 2004 Feb 19;41(4):625-38
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/28/2015
MGI 5.22
The Jackson Laboratory