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Targeted Allele Detail
Symbol: Dlg4tm2.1Grnt
Name: discs large MAGUK scaffold protein 4; targeted mutation 2.1, Seth G N Grant
MGI ID: MGI:3848928
Synonyms: PSD-95GK
Gene: Dlg4  Location: Chr11:70017203-70045535 bp, + strand  Genetic Position: Chr11, 42.96 cM, cytoband B4
Germline Transmission:  Earliest citation of germline transmission: J:89778
Parent Cell Line:  E14TG2aIV (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA STOP codon and coding sequence for haemaglutinin (HA) epitope tag were inserted in frame before the start of the GK domain at amino acid 500. In addition, a floxed neo cassette was inserted downstream to replace sequence and this cassette was subsequently excised by Cre leaving a single loxP site after the STOP codon. Western blot and immunoprecipitation analysis confirmed the absence of protein in hippocampal extracts from homozygous mice. RT-PCR analysis indicated that a very low level of mRNA expression (less than 5% of wild-type) was present in hippocampal extracts from homozygous mice. (J:149508)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dlg4 Mutation:  25 strains or lines available
Original:  J:89778 Yao WD, et al., Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticity. Neuron. 2004 Feb 19;41(4):625-38
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory