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Hprttm1(CAG-Tgfbr1*)Lba
Targeted Allele Detail
Nomenclature
Symbol: Hprttm1(CAG-Tgfbr1*)Lba
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 1, Laurent Bartholin
MGI ID: MGI:3846738
Synonyms: LSL-TbetaRICA, StopfloxTgfbRICA, TbetaRICA, TGFbetaRICA
Gene: Hprt  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:148747
Parent Cell Line:  BPES (ES Cell)
Strain of Origin:  (129-Hprtb-m3 x C57BL/6)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Constitutively active, Inserted expressed sequence)
Mutation:    Insertion
 
Hprttm1(CAG-Tgfbr1*)Lba expresses 1 gene
 
Mutation detailsCoding sequence for a constitutively active form of murine TGFbeta receptor I was placed downstream of a CAG promoter and a loxP-STOP-loxP motif. An HA tag was included on the C-terminus end followed by the first three exons of the Hprt locus to allow for correct targeting and selection in ES cells that had a deletion of the 5' end of the Hprt locus. The knocked-in Tgfbr1 sequence encoded three missense mutations: T204D that constitutively activates the TbRI kinase and L193A/P194A , mutations that prevent binding of the TbRI inhibitor, FKBP12. Expression of the gene can only be achieved by cre-mediated removal of the floxed STOP cassette. (J:148747)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1274 strains or lines available
References
Original:  J:148747 Bartholin L, et al., Generation of mice with conditionally activated transforming growth factor beta signaling through the TbetaRI/ALK5 receptor. Genesis. 2008 Dec;46(12):724-31
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory