Ascl2^tm1.1Cle
Targeted Allele Detail

Summary

• Symbol: Ascl2^tm1.1Cle
• Name: achaete-scute family bHLH transcription factor 2; targeted mutation 1.1 Hans Clevers
• MGI ID: MGI:3846671
• Synonyms: Ascl2^floxed
• Gene: Ascl2 Location: Chr7:142520566-142523001 bp, - strand Genetic Position: Chr7, 88.08 cM
• Alliance: Ascl2^tm1.1Cle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148715
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A FRT-flanked neomycin cassette and a loxP site were placed in the non-coding exon 1 with an additional loxP site placed downstream of exon 2. The neomycin cassette was removed by crossing mice with a Flipase transgenic strain leaving the lone coding exon flanked by loxP sites. An Xho site is destroyed in the introduction of the FRT and loxP sites to exon 1. Restriction digests on cDNA from heterozygote placenta and intestinal extracts indicate that the gene is imprinted in placental tissue (the maternal allele is expressed) while there is no imprinting in intestinal stem cells (both alleles are expressed). (J:148715)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ascl2 Mutation: 15 strains or lines available

References

• Original: J:148715 van der Flier LG, et al., Transcription factor achaete scute-like 2 controls intestinal stem cell fate. Cell. 2009 Mar 6;136(5):903-12
• All: 7 reference(s)