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Tg(Six2-EGFP/cre)1Amc
Transgene Detail
Nomenclature
Symbol: Tg(Six2-EGFP/cre)1Amc
Name: transgene insertion 1, Andrew P McMahon
MGI ID: MGI:3845228
Synonyms: Six2-TGCtg, Six2-TGC(tg), Tet-off-eGFPCre BAC transgenic, TGC BAC transgenic allele, Tg(Six2-tTA,tetO-EGFP/cre)1Amc
Transgene: Tg(Six2-EGFP/cre)1Amc  Location: unknown  
Transgene
origin
Strain of Origin:  CD-1
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Mutation:    Insertion
 
Mutation detailsThe 181 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RPCI23-311C1, containing the entire Six2 locus (and other genes), was modified by targeting a Tet-off-eGFPCre into the ATG start site of the Six2 locus. This inserted a Tet-off cassette (tetracycline-regulated transactivator (tTA), 2xpolyA signal, tetracycline-responsive element (TRE or tetO with CMV min promoter) followed by an Enhanced Greed Fluorescent Protein/Cre Recombinase (EGFP/Cre) fusion protein coding sequence, (SV40 polyA signal), and frt-flanked kanamycin cassette. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into CD-1 zygotes. Sequence updates published after this transgene was created revealed an upstream Six2 ATG start site that is in-frame with the tTA; thus an apparent and unintended fusion was created with the tTA (80 amino acids potentially added) that renders transgene expression unaffected by tetracycline/doxycycline administration. (J:148455)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Six2     Summary of all recombinase alleles driven by Six2.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 53 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:148455 Kobayashi A, et al., Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell. 2008 Aug 7;3(2):169-81
All:  53 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory