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Targeted Allele Detail
Symbol: Cpn1tm1Raw
Name: carboxypeptidase N, polypeptide 1; targeted mutation 1, Rick A Wetsel
MGI ID: MGI:3844631
Gene: Cpn1  Location: Chr19:43956307-43986551 bp, - strand  Genetic Position: Chr19, 36.68 cM, cytoband D2
Germline Transmission:  Earliest citation of germline transmission: J:148315
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129S1/SvImJ
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 3 of the locus was replaced by a neomycin resistance cassette. RT-PCR analysis on homozygous liver extracts detected the presence of a truncated mRNA message corresponding to a splice variant that fused exon 2 to exon 4. Because exon 3 encoded a substrate binding site, it was hypothesized that any mutant protein transcribed from the truncated mRNA would lack carboxypeptidase activity. This was confirmed by a lack of terminal arginine cleavage on C3a and C5a complement proteins. (J:148315)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cpn1 Mutation:  11 strains or lines available
ES cell line = EZ-1
Original:  J:148315 Mueller-Ortiz SL, et al., Targeted disruption of the gene encoding the murine small subunit of carboxypeptidase N (CPN1) causes susceptibility to C5a anaphylatoxin-mediated shock. J Immunol. 2009 May 15;182(10):6533-9
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory