Scpep1^{Gt(RST426)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Scpep1^{Gt(RST426)Byg}
• Name: serine carboxypeptidase 1; gene trap RST426, BayGenomics
• MGI ID: MGI:3843010
• Gene: Scpep1 Location: Chr11:88814846-88846268 bp, - strand Genetic Position: Chr11, 54.34 cM
• Alliance: Scpep1^{Gt(RST426)Byg} page

Mutation origin

• Mutant Cell Line: RST426
• Germline Transmission: Earliest citation of germline transmission: J:147885
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector, containing a beta-geo cassette, inserted into intron 7. This insertion prevents transcription of exons 8 through 13 that code for two amino acids in the putative catalytic triad. The absence of the endogenous transcript and expression of the fusion moiety was confirmed by western blot analysis. (J:147885)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST426	CC200344 ; (MGI Seq Detail)	5' RACE	Chr11:88827840-88843380 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  290 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scpep1 Mutation: 62 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:147885 Kollmann K, et al., Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1. FEBS J. 2009 Mar;276(5):1356-69
• All: 4 reference(s)