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Targeted Allele Detail
Symbol: Pou4f2tm2.1Nat
Name: POU domain, class 4, transcription factor 2; targeted mutation 2.1, Jeremy Nathans
MGI ID: MGI:3842365
Synonyms: Brn3bCKOAP
Gene: Pou4f2  Location: Chr8:79159639-79163274 bp, - strand  Genetic Position: Chr8, 36.99 cM, cytoband F1-F5
Germline Transmission:  Earliest citation of germline transmission: J:147350
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsThe locus was targeted to have its entire coding region floxed and to express a reporter gene after cre-mediated recombination. A loxP site was inserted in the upstream untranslated region 98 base pairs before the initiator ATG codon. Three repeats of the SV40 early region transcription terminator were added to the untranslated region 340 base pairs downstream of the termination codon, followed by a second loxP site and the coding region of human placental alkaline phosphatase (AP). A frt-flanked neomycin selection cassette followed the AP coding region and was subsequently removed by crossing to mice expressing FLP recombinase. Following cre-mediated recombination, AP activity is detectable in the retina and brain. (J:147350)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou4f2 Mutation:  6 strains or lines available
Original:  J:147350 Badea TC, et al., Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells. Neuron. 2009 Mar 26;61(6):852-64
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory