Foxo1^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Foxo1^tm1Flv
• Name: forkhead box O1; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:3840910
• Synonyms: Foxo1^flox
• Gene: Foxo1 Location: Chr3:52175758-52257530 bp, + strand Genetic Position: Chr3, 23.19 cM
• Alliance: Foxo1^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147057
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into the XbaI restriction site upstream of exon 1 and a frt-flanked neoymycin cassette with a 3' loxP site was inserted downstream of exon 1. Mice were crossed with transgenic Flipase to remove the neomycin cassette, leaving exon 1 floxed. Protein expression was unaffected as determined by immunoblot analysis of homozygote lymphocyte extracts. (J:147057)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Foxo1 Mutation: 31 strains or lines available

References

• Original: J:147057 Ouyang W, et al., An essential role of the Forkhead-box transcription factor Foxo1 in control of T cell homeostasis and tolerance. Immunity. 2009 Mar;30(3):358-71
• All: 14 reference(s)