Asxl2^{Gt(AQ0356)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Asxl2^{Gt(AQ0356)Wtsi}
• Name: ASXL transcriptional regulator 2; gene trap AQ0356, Wellcome Trust Sanger Institute
• MGI ID: MGI:3840841
• Gene: Asxl2 Location: Chr12:3476857-3556852 bp, + strand Genetic Position: Chr12, 1.81 cM
• Alliance: Asxl2^{Gt(AQ0356)Wtsi} page

Axial skeleton patterning is disrupted in Asxl2^{Gt(AQ0356)Wtsi}/Asxl2^{Gt(AQ0356)Wtsi} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AQ0356
• Germline Transmission: Earliest citation of germline transmission: J:147372
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector was inserted 5016 bp downstream of exon 1. RT-PCR confirmed the expression of an fusion transcript between exon 1 and the gene trap vector. Real time PCR detected expression of wild type transcript in hearts from homozygous mice at about 3% of the level seen in wild-type hearts. LacZ expression was detected in the developing heart, axial skeleton, neocortex, spinal cord, and eye. Postnatally expression was seen in the heart, retina, ovary and testis. (J:147372)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AQ0356	CZ259004 ; (MGI Seq Detail)	5' RACE	Chr12:3477039-3477211 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  84 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Asxl2^{Gt(AQ0356)Wtsi} allele

Expression

In Mice Carrying this Mutation:	31 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asxl2 Mutation: 124 strains or lines available

References

• Original: J:147372 Baskind HA, et al., Functional conservation of asxl2, a murine homolog for the Drosophila enhancer of trithorax and polycomb group gene asx. PLoS One. 2009;4(3):e4750
• All: 8 reference(s)