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Targeted Allele Detail
Symbol: Selltm3Hyn
Name: selectin, lymphocyte; targeted mutation 3, Richard Hynes
MGI ID: MGI:3839977
Synonyms: L-selectin-
Gene: Sell  Location: Chr1:164061987-164082930 bp, + strand  Genetic Position: Chr1, 71.37 cM
Germline Transmission:  Earliest citation of germline transmission: J:57973
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. To generate double knockouts, ES cells heterozygote for the Selptm1Hyn and Seletm2Hyn null alleles in trans to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis of homozygote leukocytes showed an absence of the L-selectin and P-selectin protein while E-selectin expression was unaffected. Thus, this targeted mutation occurred in cis with the Selptm1Hyn allele and in trans with the Seletm2Hyn allele. Due to their genomic proximity, the Selltm3Hyn and Selptm1Hyn alleles should propagate together through the offspring. (J:57973)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Sell Mutation:  30 strains or lines available
Original:  J:57973 Robinson SD, et al., Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11452-7
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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