Gabbr2^tm1Arca
Targeted Allele Detail

Summary

• Symbol: Gabbr2^tm1Arca
• Name: gamma-aminobutyric acid type B receptor subunit 2; targeted mutation 1, Andrew R Calver
• MGI ID: MGI:3839235
• Synonyms: deltaGB2-Ct
• Gene: Gabbr2 Location: Chr4:46662318-46991714 bp, - strand Genetic Position: Chr4, 25.18 cM
• Alliance: Gabbr2^tm1Arca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146953
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 14 and 15 were replaced with a PGK neomycin selection cassette. The PGK neo cassette is spliced out during mRNA production and exon 13 is fused to exon 16 in the resulting mRNA. An additional six amino acids are added downstream of transmembrane domane 7. Expression of the mutant mRNA was confirmed by quantitative RT-PCR. (J:146953)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabbr2 Mutation: 61 strains or lines available

References

• Original: J:146953 Thuault SJ, et al., The GABA(B2) subunit is critical for the trafficking and function of native GABA(B) receptors. Biochem Pharmacol. 2004 Oct 15;68(8):1655-66
• All: 1 reference(s)