Igh-7^tm1Lam
Targeted Allele Detail

Summary

• Symbol: Igh-7^tm1Lam
• Name: immunoglobulin heavy chain 7 (heavy chain of IgE); targeted mutation 1, Marinus C Lamers
• MGI ID: MGI:3838714
• Synonyms: KVKdelta-tail
• Gene: Igh-7 Location: Chr12:113228142-113241336 bp Genetic Position: Chr12, Syntenic
• Alliance: Igh-7^tm1Lam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146733
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A point mutation was targeted to the third codon of exon 6 to introduce a stop codon and a frameshift mutation to the portion of the gene that encodes the cytoplasmic tail. LoxP sites were also inserted upstream of exon 5 and downstream of the mutated exon 6. A neomycin/tk selection cassette followed by a third loxP site was inserted further downstream. Transient expression of cre recombinase removed the selection cassette while leaving the mutated exon 6 in place. Correct targeting and recombination were confirmed by Southern blotting. This allele was predicted to encode an IgE heavy chain with a cytoplasmic tail only 3 amino acids long (KVK) that was identical to the cytoplasmic domain of mIgM and mIgD. (J:146733)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-7 Mutation: 1 strain or line available

References

• Original: J:146733 Achatz G, et al., Effect of transmembrane and cytoplasmic domains of IgE on the IgE response. Science. 1997 Apr 18;276(5311):409-11
• All: 1 reference(s)