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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm1(CAG-Kcnj11*,-GFP)Nich
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Colin G Nichols
MGI ID: MGI:3838361
Synonyms: mutKir6.2, Rosa26-Kir6.2, Rosa26-Kir6.2(K185Q,deltaN30)
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077333 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:146650
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
Gt(ROSA)26Sortm1(CAG-Kcnj11*,-GFP)Nich expresses 1 gene
Mutation detailsThe targeting construct contained the CAG promoter (chicken beta actin promoter with CMV enhancers), a floxed neomycin-Westphal stop sequence (WSS) cassette, a modified Kcnj11 gene, an IRES and GFP sequence. Kcnj11 has been modified by a 30 amino acid N-terminal truncation and by a modification of the codon for amino acid residue 185 causing a lysine to glutamine substitution. Cre mediated excision of the neo-WSS cassette permits expression of the mutated Kcnj11 and GFP under the control of the CAG promoter. (J:146650)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  403 strains or lines available
Original:  J:146650 Remedi MS, et al., Secondary consequences of beta cell inexcitability: identification and prevention in a murine model of K(ATP)-induced neonatal diabetes mellitus. Cell Metab. 2009 Feb;9(2):140-51
All:  7 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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