Ighg1^tm2Cgn
Targeted Allele Detail

Summary

• Symbol: Ighg1^tm2Cgn
• Name: immunoglobulin heavy constant gamma 1 (G1m marker); targeted mutation 2, University of Cologne
• MGI ID: MGI:3837936
• Synonyms: IgH^{delta5'sgamma1a}, IgH^{neodelta5'sgamma1a}
• Gene: Ighg1 Location: Chr12:113290161-113294143 bp, - strand Genetic Position: Chr12, 62.1 cM
• Alliance: Ighg1^tm2Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146428
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An inversely oriented FRT-flanked neomycin cassette replaced 1.7 kb of the 5' sgamma1 switch region that is upstream of the IgG1 constant region and includes sites of DNA modification and a splice donor site. After targeting 129P2/OlaHsd ES cells, the neomycin cassette was removed by transient expression of Flpe recombinase. Inactivation of the IgG1 switch region was confirmed in (C57BL/6 x 129P2/OlaHsd)F1 mice by a lack of IgG1 with the "a" 129 haplotype (i.e. IgG1^a). (J:146428)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ighg1 Mutation: 28 strains or lines available

References

• Original: J:146428 Jung S, et al., Shutdown of class switch recombination by deletion of a switch region control element. Science. 1993 Feb 12;259(5097):984-7
• All: 4 reference(s)