Drinkcacl21^PWK/PhJ
QTL Variant Detail

Summary

• QTL variant: Drinkcacl21^PWK/PhJ
• Name: drink calcium chloride 2 1; PWK/PhJ
• MGI ID: MGI:3837198
• QTL: Drinkcacl21 Location: unknown Genetic Position: Chr4, Syntenic

Variant origin

• Strain of Specimen: PWK/PhJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased preference for calcium chloride compared to C57BL/6J. (J:144262)
• Inheritance: Other (see notes)

Notes

Drinkcacl21 exhibits additive inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144262

Linkage analysis was performed on (C57BL/6J x PWK/PhJ)F2 animals to identify genetic loci associated with preference and intake of calcium and other salt compounds. Animals were subjected to a two-bottle choice test between water and one other solution (CaCl2, CaLa, MgCl2, KCl, NH4Cl, NaCl, citric acid, QHCl, or saccharin) for a period of 96 hours. PWK/PhJ is a high consumer of calcium chloride (CaCl2) and calcium lactate (CaLa) whereas C57BL/6J is a relatively low consumer. PWK/PhJ also consumes more MgCl2 compared to C57BL/6J. However, when consumption of KCl, NaCl and saccharin were analyzed, C57BL/6J animals consumed more of these solutions compared to PWK/PhJ animals. Linkage analysis was performed using 116 polymorphic markers at a resolution of 10cM - 30cM. Thirty QTLs reaching statistical significance were identified. Authors assigned separate QTL nomenclature for each of the salt compound QTL even if they mapped to the same linkage marker.

On mouse Chromosome 4, significant linkage to CaCl2, CaLa, MgCl2 and saccharin preference mapped to 83 cM (155 Mb) near rs13478075. These loci are named Drinkcacl21 (drink calcium chloride 2 1; LOD=5.7), Drinkcala1 (drink calcium lactate 1; LOD=6.8), Drinkmgcl21 (drink magnesium chloride 2; LOD=12.6), and Drinksac1 (drink saccharin 1; LOD=8.9). PWK/PhJ-derived alleles at Drinkcacl21 and Drinkcala1 confer increased calcium preference with an additive mode of inheritance. Drinkcacl21 explains 7.1% of the variance for CaCl2 preference and Drinkcala1 explains 10.1% of the variance for CaLa preference. Drinkmgcl21 is also linked to MgCl2 intake with LOD=6.7 and Drinksac1 is also linked to saccharin intake with LOD=11.6. PWK/PhJ-derived alleles at Drinkmgcl21 confer increased MgCl2 intake and preference with additive inheritance. C57BL/6J-derived alleles at Drinksac1 confer increased saccharin intake and preference with a dominant mode of inheritance. Drinkmgcl21 explains 6.7% and 12.6% of the variance for MaCl2 intake and preference, respectively, and Drinksac1 explains 10.9% and 10.3% of the variance for saccharin intake and preference, respectively. Authors name Tas1r3 at 83 cM as a strong candidate gene for Drinksac1, Drinkcacl21, Drinkcala1 and Drinkmgcl21.

References

• Original: J:144262 Tordoff MG, et al., Calcium taste preferences: genetic analysis and genome screen of C57BL/6J x PWK/PhJ hybrid mice. Genes Brain Behav. 2008 Aug;7(6):618-28
• All: 1 reference(s)