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Transgene Detail
Symbol: Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli
Name: transgene insertion 1, Jack A Elias
MGI ID: MGI:3836057
Synonyms: CC10-rtTA-tTS-TGF-beta1 Tg, CC10-tTS-rtTA-TGF-beta1 Tg, TgN(CC10-rtTA)-TgN(tTS)-TgN(TGF-b1)
Transgene: Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli  Location: unknown  
Strain of Origin:  Not Specified
Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Transactivator)
Mutation:    Insertion
Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli expresses 1 gene
Mutation detailsThe CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivator (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-Tgfb1-SV40, contains a polymeric tetracycline operator (tet-O), minimal cytomegalovirus (CMV) promoter, human Tgfb1 cDNA in which the the cysteine codons at positions 223 and 225 were replaced with serine codons, and SV40 polyadenylation signals. The third construct, CC10-tTS-hGH, has the CC10 promoter driving the expression of the tetracycline-controlled transcriptional silencer (tTS). All three constructs were coinjected into fertilized oocytes, and triple positive founder animals were obtained. In this system, the CC10 promoter constitutively drives the expressionof rtTA and tTS in a lung-specific fashion. In the absence of dox, tTS binds to and actively suppresses the expression of the tet-Oregulated TGF-beta1 transgene. In the presence of dox, tTS is released allowing the activating, dox binding rtTA to bind the tet-O and activate transgene expression. (J:92469)
View phenotypes and curated references for all genotypes (concatenated display).
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Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:92469 Lee CG, et al., Early growth response gene 1-mediated apoptosis is essential for transforming growth factor beta1-induced pulmonary fibrosis. J Exp Med. 2004 Aug 2;200(3):377-89
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
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