Hprt1^{tm16(Ple167-EGFP/cre)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm16(Ple167-EGFP/cre)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 16, Elizabeth M Simpson
• MGI ID: MGI:3835530
• Synonyms: Hprt1^{tm16(mEMS681)Ems}, Hprt1^{tm16(Ple167-EGFPcre;mEMS681)Ems}, NLS-EGFP/cre, Ple167, POGZ-A-EGFP/cre
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm16(Ple167-EGFP/cre)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS668, mEMS670, mEMS672, mEMS681
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Hprt1^{tm16(Ple167-EGFP/cre)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  POGZ (23126)

  Pogz	(MGI:2442117)

• Mutation details: The Ple167-EGFP/cre transgene (pEMS1091) was designed with the 1200 bp Ple167 minipromoter (POGZ-A; derived from a portion of the promoter of the POGZ gene from the Pogo transposable element with ZNF domain (POGZ) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. (J:144244, J:164356)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	POGZ (human) Summary of all recombinase alleles driven by POGZ. Comparative matrix view of recombinase activities Alliance human POGZ gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS681 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 4 reference(s)