Hprt1^{tm14(Ple103-EGFP/cre)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm14(Ple103-EGFP/cre)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 14, Elizabeth M Simpson
• MGI ID: MGI:3835525
• Synonyms: HAP1-EGFP/cre, Hprt1^{tm14(mEMS675)Ems}, Hprt1^{tm14(Ple103-EGFPcre;mEMS675)Ems}, Ple103
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm14(Ple103-EGFP/cre)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS675, mEMS676, mEMS689, mEMS1031
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Hprt1^{tm14(Ple103-EGFP/cre)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  HAP1 (9001)

  Hap1	(MGI:1261831)

• Mutation details: The Ple103-EGFP/cre transgene (pEMS1083) was designed with the 877 bp Ple103 minipromoter (HAP1-A; derived from a segment of the basal promoter element of the human huntingtin-associated protein 1 (HAP1) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprts^b-m3 mutant locus on the X chromosome. (J:144244, J:164356)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	HAP1 (human) Summary of all recombinase alleles driven by HAP1. Comparative matrix view of recombinase activities Alliance human HAP1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS675 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)