Hprt1tm10(Ple162-EGFP/cre)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm10(Ple162-EGFP/cre)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 10, Elizabeth M Simpson |
MGI ID: |
MGI:3835521 |
Synonyms: |
Hprt1tm10(mEMS954)Ems, Hprt1tm10(Ple162-EGFPcre;mEMS954)Ems, PITX3-EGFP/cre |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Alliance: |
Hprt1tm10(Ple162-EGFP/cre)Ems page
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Mutant Cell Lines: |
mEMS951, mEMS953, mEMS954 |
Germline Transmission: |
Earliest citation of germline transmission:
J:145689
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Project Collection: |
Pleiades Promoter Project
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Allele Type: |
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Targeted (Recombinase, Reporter) |
Mutation: |
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Insertion
Vector Type: Not Applicable
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Hprt1tm10(Ple162-EGFP/cre)Ems expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Homolog in Mouse |
Note |
human |
PITX3 (5309) |
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Mutation details: The Ple162-EGFPCre transgene (pEMS1148) was designed with the 3636 bp Ple162 minipromoter (PITX3-D; derived from an intronic candidate regulatory region and a segment of the human paired-like homeodomain 3 (PITX3) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2).
(J:164356)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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PITX3
(human)
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View phenotypes and curated references for all genotypes (concatenated display).
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Germ line transmission of mutant cell line mEMS954 has been established.
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Original: |
J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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