Tg(GFAP-Ccre)FFki
Transgene Detail

Summary

• Symbol: Tg(GFAP-Ccre)FFki
• Name: transgene insertion F, Frank Kirchhoff
• MGI ID: MGI:3833400
• Synonyms: GCCF, GFAP-CCre, Tg(GFAP-icre*)FFki
• Transgene: Tg(GFAP-Ccre)FFki Location: unknown
• Alliance: Tg(GFAP-Ccre)FFki page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(GFAP-Ccre)FFki expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  GFAP (2670)

  Gfap	(MGI:95697)

• Mutation details: The coding sequence for improved cre recombinase was split into two complementary halves. For this transgene construct, the c-terminal half of the cre gene encoding for amino acids 60-343 was fused on its 5' end to an element encoding the coiled-coil leucine zipper domain of the yeast transcription factor GCN4. A myc tag was also included on the 5' end of the fused construct. This construct was placed under control of the human GFAP promoter to drive expression in astrocytes. When this transgene is co-expressed with a transgene that expresses the n-terminal half of icre fused to a leucine zipper domain, the leucine zippers force dimerization resulting in a functional cre recombinase. This is the only line created in the original study. (J:144851)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	GFAP (human) Summary of all recombinase alleles driven by GFAP. Comparative matrix view of recombinase activities Alliance human GFAP gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:144851 Hirrlinger J, et al., Split-cre complementation indicates coincident activity of different genes in vivo. PLoS One. 2009;4(1):e4286
• All: 1 reference(s)