Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Frances M Ashcroft
• MGI ID: MGI:3832575
• Synonyms: floxed Kir6.2-V59M
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas} page

Reduction in islet insulin content in Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas}/Gt(ROSA)26Sor⁺ Tg(Ins2-cre)23Herr/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144715
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kcnj11 (MGI:107501)

• Mutation details: A DNA fragment was replaced by a construct consisting of a splice acceptor, a floxed PGK-neo stop cassette, a mouse genomic DNA encoding Kcnj11 with a V59M mutation, and an frt flanked IRES GFP cassette via homologous recombination. Cre mediated removal of the floxed stop cassette allows for expression of Kcnj11. (J:144715)

Generation of the Gt(ROSA)26Sor^{tm1(Kcnj11*V59M)Fmas} allele

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:144715 Girard CA, et al., Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic beta cells recapitulates neonatal diabetes. J Clin Invest. 2009 Jan;119(1):80-90
• All: 14 reference(s)