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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm4(HIF2A*)Kael
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, William G Kaelin
MGI ID: MGI:3832386
Synonyms: Epas1LSL, HIF2dPA, LSL-HIF2dPA, ROSA26-HA-HIF2dPA
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm4(HIF2A*)Kael page
Germline Transmission:  Earliest citation of germline transmission: J:144666
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
Mutations:    Insertion, Nucleotide substitutions
Gt(ROSA)26Sortm4(HIF2A*)Kael expresses 1 gene
Mutation detailsDNA encoding influenza virus hemagglutinin A- (HA-) tagged human hypoxia-inducible factor 2 alpha (HIF2alpha) with nucleotide substitutions leading to replacement of proline by alanine at amino acid positions 405 and 531 (P405A;P531A), preceded by a floxed neo-STOP cassette, was inserted into the targeted locus. The STOP sequence must be removed by Cre-mediated recombination to allow for expression of the mutated coding sequence. The amino acid substitutions prevent oxygen-dependent prolyl hydroxylation at a.a. position 405 or 531, which is required for binding by von Hippel-Lindau tumor suppressor (VHL). (J:144666)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  784 strains or lines available
Original:  J:144666 Kim WY, et al., Failure to prolyl hydroxylate hypoxia-inducible factor alpha phenocopies VHL inactivation in vivo. EMBO J. 2006 Oct 4;25(19):4650-62
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory