Cdc25a^tm1Dvb
Targeted Allele Detail

Summary

• Symbol: Cdc25a^tm1Dvb
• Name: cell division cycle 25A; targeted mutation 1, Dmitry V Bulavin
• MGI ID: MGI:3831687
• Synonyms: Cdc25A^S123A
• Gene: Cdc25a Location: Chr9:109704647-109722963 bp, + strand Genetic Position: Chr9, 59.81 cM
• Alliance: Cdc25a^tm1Dvb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144548
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A serine 123 to alanine (S123A) substitution was performed by mutating the TCT codon in exon 5 to GCT. A loxP-flanked neomycin cassette was also included upstream of exon 5 for targeting purposes. Mice generated by correctly targeted ES cells were crossed with cre-transgenic mice to remove the neo cassette leaving behind a single loxP site. Sequence analysis of cDNAs from homozygote embryonic fibroblasts demonstrated the mutated allele was expressed. Immunoblot analysis indicated no differences in basal expression between mutant and wild-type proteins. The mutant protein was found to have a 50% longer half-life than wild-type protein in embryonic fibroblasts. (J:144548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdc25a Mutation: 34 strains or lines available

References

• Original: J:144548 Shreeram S, et al., Cdc25A serine 123 phosphorylation couples centrosome duplication with DNA replication and regulates tumorigenesis. Mol Cell Biol. 2008 Dec;28(24):7442-50
• All: 1 reference(s)