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Cdc25atm1Dvb
Targeted Allele Detail
Nomenclature
Symbol: Cdc25atm1Dvb
Name: cell division cycle 25A; targeted mutation 1, Dmitry V Bulavin
MGI ID: MGI:3831687
Synonyms: Cdc25AS123A
Gene: Cdc25a  Location: Chr9:109704647-109722963 bp, + strand  Genetic Position: Chr9, 59.81 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:144548
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted (Not Specified)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsA serine 123 to alanine (S123A) substitution was performed by mutating the TCT codon in exon 5 to GCT. A loxP-flanked neomycin cassette was also included upstream of exon 5 for targeting purposes. Mice generated by correctly targeted ES cells were crossed with cre-transgenic mice to remove the neo cassette leaving behind a single loxP site. Sequence analysis of cDNAs from homozygote embryonic fibroblasts demonstrated the mutated allele was expressed. Immunoblot analysis indicated no differences in basal expression between mutant and wild-type proteins. The mutant protein was found to have a 50% longer half-life than wild-type protein in embryonic fibroblasts. (J:144548)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cdc25a Mutation:  26 strains or lines available
References
Original:  J:144548 Shreeram S, et al., Cdc25A serine 123 phosphorylation couples centrosome duplication with DNA replication and regulates tumorigenesis. Mol Cell Biol. 2008 Dec;28(24):7442-50
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory