Id1^tm1Xhsu
Targeted Allele Detail

Summary

• Symbol: Id1^tm1Xhsu
• Name: inhibitor of DNA binding 1, HLH protein; targeted mutation 1, Xiao-Hong Sun
• MGI ID: MGI:3831561
• Synonyms: Id1^GFP
• Gene: Id1 Location: Chr2:152578171-152579330 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Id1^tm1Xhsu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144696
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An eGFP vector and a floxed neomycin cassette was knocked into the untranslated region of exon 1 of the gene locus. Founder mice were crossed with EIIa-cre transgenic mice to remove the neomycin cassette, leaving behind a loxP site. GFP expression was detected in bone marrow cells of myeloid lineages in both heterozygote and homozygote mice. Endogenous protein was not detected in immunobloting of cytokine-stimulated bone marrow cells from homozygote mice confirming this is a null allele. (J:144696)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Id1 Mutation: 8 strains or lines available

References

• Original: J:144696 Perry SS, et al., Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance. Blood. 2007 Oct 1;110(7):2351-60
• All: 9 reference(s)