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Targeted Allele Detail
Symbol: Fgf2tm3Doe
Name: fibroblast growth factor 2; targeted mutation 3, Thomas Doetschman
MGI ID: MGI:3831082
Synonyms: Fgf2hmw-ko, FGF2(Lo)
Gene: Fgf2  Location: Chr3:37402616-37464255 bp, + strand  Genetic Position: Chr3, 18.41 cM, cytoband A2-B
Germline Transmission:  Earliest citation of germline transmission: J:144353
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Modified isoform(s))
Mutations:    Insertion, Intragenic deletion
Mutation detailsUsing a modified Tag & Exchange targeting scheme, the Hprt minigene in the tagged Fgf2 knock-out allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a 14 bp oligonucleotide designed to produce stop codons in all three reading frames and to cause a frameshift in translation products originating from the noncanonical CTG initiation codon. Cells with the exchanged allele were selected for the loss of HPRT function using 6-thioguanine. The loss of high molecular weight protein product expression was confirmed by western blot analysis on heart, liver and brain extracts. The low molecular weight protein product is still expressed. (J:144353)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fgf2 Mutation:  14 strains or lines available
Original:  J:144353 Azhar M, et al., Gene targeted ablation of high molecular weight fibroblast growth factor-2. Dev Dyn. 2009 Feb;238(2):351-7
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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