Cpa3^tm3(icre)Hrr
Targeted Allele Detail

Summary

• Symbol: Cpa3^tm3(icre)Hrr
• Name: carboxypeptidase A3, mast cell; targeted mutation 3, Hans-Reimer Rodewald
• MGI ID: MGI:3829878
• Synonyms: Cpa3^Cre, Cpa3^tm3Hrr
• Gene: Cpa3 Location: Chr3:20269784-20296345 bp, - strand Genetic Position: Chr3, 6.25 cM
• Alliance: Cpa3^tm3(icre)Hrr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143731
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Cpa3^tm3(icre)Hrr expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cpa3 (MGI:88479)

• Mutation details: The coding sequence for the first 28 nucleotides of the gene locus was replaced with a codon-improved cre recombinase expression cassette and a Frt-flanked neomycin selection cassette. Transient expression of Flp-recombinase in correctly targeted ES cells removed the selection cassette. When mice carrying this cre knock-in allele were crossed with mice carrying the cre-dependent reporter allele Gt(ROSA)26Sor^tm1Hjf, reporter expression was observed in about 25% of early pro-T cells in the thymus. By the DN2 stage (CD44⁺CD25⁺CD4^-CD8^-) of T cell development, 90% of the cells expressed the reporter as did 90% of peripheral T cells. A small percentage (<5%) of mature B cells also expressed the reporter. Bone marrow analysis demonstrated expression in some (10-30%) stem cells, common lymphoid progenitors and in myeloid- and plasmacytoid- dendritic cells. (J:143731, J:178942)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cpa3 (mouse) Summary of all recombinase alleles driven by Cpa3. Alliance mouse Cpa3 gene page

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Cpa3^tm3(icre)Hrr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpa3 Mutation: 30 strains or lines available

References

• Original: J:143731 Feyerabend TB, et al., Deletion of Notch1 converts pro-T cells to dendritic cells and promotes thymic B cells by cell-extrinsic and cell-intrinsic mechanisms. Immunity. 2009 Jan;30(1):67-79
• All: 27 reference(s)