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Targeted Allele Detail
Symbol: Nfkb1tm1Sley
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105; targeted mutation 1, Steven C Ley
MGI ID: MGI:3822895
Synonyms: Nfkb1S927A,S932A, Nfkb1SSAA/SSAA
Gene: Nfkb1  Location: Chr3:135584655-135691547 bp, - strand  Genetic Position: Chr3, 62.82 cM
Germline Transmission:  Earliest citation of germline transmission: J:142340
Parent Cell Line:  PC3 (ES Cell)
Strain of Origin:  129S4/SvJae-Tg(Prm-cre)70Og
Allele Type:    Targeted (Not Specified)
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsA targeting vector was designed to substitute alanine for serine at positions 927 and 933 (S927A, S932A). Targeting took place in PC3 ES cells in which Cre recombinase expression is restricted to haploid spermatids. A loxP-flanked neomycin resistance cassette was included upstream of exon 24 for targeting purposes and was subsequently removed by Cre mediated recombination upon transmission through male chimeric mice. This mutation was predicted to prevent phosphorylation of the gene product by IKK making it resistant to signal-induced degradation. (J:142340)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nfkb1 Mutation:  91 strains or lines available
PC3 ES cells are homozygous for Tg(Prm-cre)70Og.
Original:  J:142340 Sriskantharajah S, et al., Proteolysis of NF-kappaB1 p105 is essential for T cell antigen receptor-induced proliferation. Nat Immunol. 2009 Jan;10(1):38-47
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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