Shmt1^tm1Stov
Targeted Allele Detail

Summary

• Symbol: Shmt1^tm1Stov
• Name: serine hydroxymethyltransferase 1 (soluble); targeted mutation 1, Patrick J Stover
• MGI ID: MGI:3821629
• Synonyms: Shmt1^flox
• Gene: Shmt1 Location: Chr11:60678933-60702091 bp, - strand Genetic Position: Chr11, 37.84 cM
• Alliance: Shmt1^tm1Stov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141998
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were placed around a 355 bp fragment including 73 bp of intron 6, all of exon 7, and 69 bp of intron 7 plus an IRES betageo cassette via homologous recombination. Immunoblot analysis indicated decreased protein expression in liver, kidney, and colon from homozygous mice (J:141998)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shmt1 Mutation: 43 strains or lines available

References

• Original: J:141998 MacFarlane AJ, et al., Cytoplasmic serine hydroxymethyltransferase regulates the metabolic partitioning of methylenetetrahydrofolate but is not essential in mice. J Biol Chem. 2008 Sep 19;283(38):25846-53
• All: 1 reference(s)