Gfi1b^tm1Tmo
Targeted Allele Detail

Summary

• Symbol: Gfi1b^tm1Tmo
• Name: growth factor independent 1B; targeted mutation 1, Tarik Moroy
• MGI ID: MGI:3821577
• Synonyms: Gfi1b^GFP, Gfi1b:GFP
• Gene: Gfi1b Location: Chr2:28499462-28511994 bp, - strand Genetic Position: Chr2, 19.38 cM
• Alliance: Gfi1b^tm1Tmo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141749
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the locus was replaced by a Gfp reporter gene and a loxP-flanked neomycin resistance cassette. Chimeric mice were crossed with a cre-deleter strain to remove the neo cassette, leaving behind a single loxP site. The mutated allele has the Gfp gene inserted in-frame with the endogenous start and stop codons. Fetal livers from E13.5 homozygous embryos have expression of GFP but no expression of the endogenous gene locus, as determined by immunoblot analysis. GFP expression is visible by fluorescent microscopy in both heterozygote and homozygote E13.5 embryos. (J:141749)

Expression

In Mice Carrying this Mutation:	160 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gfi1b Mutation: 20 strains or lines available

References

• Original: J:141749 Vassen L, et al., Gfi1b:green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1. Blood. 2007 Mar 15;109(6):2356-64
• All: 9 reference(s)