Bpnt2^{Gt(RST634)Byg}
Gene trapped Allele Detail

Nomenclature

• Symbol: Bpnt2^{Gt(RST634)Byg}
• Name: 3'(2'), 5'-bisphosphate nucleotidase 2; gene trap RST634, BayGenomics
• MGI ID: MGI:3810994
• Synonyms: gPAPP^gt
• Gene: Bpnt2 Location: Chr4:4762484-4793306 bp, - strand Genetic Position: Chr4, 2.56 cM

Skeletal abnormalities of Bpnt2^{Gt(RST634)Byg}/Bpnt2^{Gt(RST634)Byg} mutant mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RST634
• Germline Transmission: Earliest citation of germline transmission: J:139706
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0TMpfs Vector Type: secretory trap
• Mutation details: Insertion of the gene-trap vector in the first intron encodes the first exon of Impad1 gene fused to beta-galactosidase. It is predicted to express a truncated mutant protein that would contain only 1 of 13 catalytically conserved residues. Western blot and biochemical analyses confirmed homozygous mice are deficient for functional enzyme. (J:139706)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RST634; (IGTC)	CC249671 ; (MGI Seq Detail)	5' RACE	Chr4:4792731-4792851 bp (-)
  * GRCm38; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;
  Blue line marks approximate position of gene trap insertion;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	28 assay results
In Structures Affected by this Mutation:	13 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Bpnt2 Mutation: 3 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:139706 Frederick JP, et al., A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11605-12
• All: 3 reference(s)