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Tg(IGH@yHG1/2)1Amgn
Transgene Detail
Summary
Symbol: Tg(IGH@yHG1/2)1Amgn
Name: transgene insertion 1, Amgen
MGI ID: MGI:3810128
Synonyms: yHG1/2
Transgene: Tg(IGH@yHG1/2)1Amgn  Location: unknown  
Alliance: Tg(IGH@yHG1/2)1Amgn page
Transgene
origin
Germline Transmission:  Earliest citation of germline transmission: J:139792
Parent Cell Line:  E14.1TG3B1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(IGH@yHG1/2)1Amgn expresses 1 gene
 
Mutation detailsA yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin heavy chain gene cluster. This YAC consisted of 66 V-heavy gene segments, all 30 D gene segments, all 6 J segments, the C-mu, and the C-delta gene segments, the human gamma2 class switch recombination recognition site, followed by the gamma 1 constant region. Downstream of this, the human exons encoding the membrane sequence of gamma2 constant regions were introduced followed by a downstream murine enhancer element. Southern blot analysis confirmed that the YAC inserted into ES cells without any recombination events. Mice carrying this transgene in combination with other alleles are capable of producing high yields of human IgG1 to an immunized antigen. (J:139792)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Notes
The Xenomouse HG1/2 transgenic strain contains this allele along with the Igh-Jtm1Jkbv, Igk-Ctm1Jkbv, Tg(IGK@yK2)J23.1Jkbv alleles.
References
Original:  J:139792 Green LL, et al., Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions. MGI Direct Data Submission. 2008;
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory