Txn2^tm1Afst
Targeted Allele Detail

Summary

• Symbol: Txn2^tm1Afst
• Name: thioredoxin 2; targeted mutation 1, A Stewart
• MGI ID: MGI:3806115
• Synonyms: Trx2^-
• Gene: Txn2 Location: Chr15:77799251-77813194 bp, - strand Genetic Position: Chr15, 36.92 cM
• Alliance: Txn2^tm1Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138283
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An Engrailed-2 splice acceptor was fused to the encephalomyocarditis virus internal ribosomal entry site (IRES) and place upstream of a lacZ-neomycin fusion reporter that had been modified by the insertion of a Tn903 promoter between the lacZ and the neo. This was followed by an SV40 polyadenylation signal. This construct was flanked by Frt sites and an additional loxP sites flank exon 2. This construct was then targeted into intron 1. (J:138283)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Txn2 Mutation: 26 strains or lines available

Notes

This allele was produced as part of a proof of principle for a targeted gene trap scheme.

References

• Original: J:138283 Testa G, et al., A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis. 2004 Mar;38(3):151-8
• All: 1 reference(s)