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Targeted Allele Detail
Symbol: Hrastm2Xbr
Name: Harvey rat sarcoma virus oncogene; targeted mutation 2, Xose R Bustelo
MGI ID: MGI:3803587
Synonyms: H-RasG12V
Gene: Hras  Location: Chr7:141189105-141194005 bp, - strand  Genetic Position: Chr7, 86.48 cM
Virgin Hrastm2Xbr/Hrastm2Xbr females display mammary gland hyperplasia

Show the 3 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:137729
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Reporter)
Mutations:    Insertion, Single point mutation
Mutation detailsIn ES cells containing Hras1tm1Xbr, a floxed hygro STOP cassette was inserted upstream of coding exon 1. In addition, a G to T substitutions was created at the second base of codon 12 of exon 1. This results in an amino acid substitution of valine for glycine. Germ line, cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12V mutation and the IRES-beta-geo cassette. (J:137729)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 26 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hras Mutation:  24 strains or lines available
This mutation was generated by re-targeting R1 ES cells in which Hras had been targeted to create Hrastm1Xbr. (J:137729)
Original:  J:137729 Schuhmacher AJ, et al., A mouse model for Costello syndrome reveals an Ang II-mediated hypertensive condition. J Clin Invest. 2008 Jun;118(6):2169-79
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.12
The Jackson Laboratory