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Targeted Allele Detail
Symbol: Hrastm2Xbr
Name: Harvey rat sarcoma virus oncogene; targeted mutation 2, Xose R Bustelo
MGI ID: MGI:3803587
Synonyms: H-RasG12V
Gene: Hras  Location: Chr7:141189105-141194005 bp, - strand  Genetic Position: Chr7, 86.48 cM
Virgin Hrastm2Xbr/Hrastm2Xbr females display mammary gland hyperplasia

Show the 3 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:137729
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Reporter)
Mutations:    Insertion, Single point mutation
Mutation detailsIn ES cells containing Hras1tm1Xbr, a floxed hygro STOP cassette was inserted upstream of coding exon 1. In addition, a G to T substitutions was created at the second base of codon 12 of exon 1. This results in an amino acid substitution of valine for glycine. Germ line, cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12V mutation and the IRES-beta-geo cassette. (J:137729)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hras Mutation:  24 strains or lines available
This mutation was generated by re-targeting R1 ES cells in which Hras had been targeted to create Hrastm1Xbr. (J:137729)
Original:  J:137729 Schuhmacher AJ, et al., A mouse model for Costello syndrome reveals an Ang II-mediated hypertensive condition. J Clin Invest. 2008 Jun;118(6):2169-79
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.11
The Jackson Laboratory