Th^{tm1(cre/Esr1)Nat}
Targeted Allele Detail

Summary

• Symbol: Th^{tm1(cre/Esr1)Nat}
• Name: tyrosine hydroxylase; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:3795871
• Synonyms: TH^CreER, TH^iCreER, TH-IRES-creER, TH-IRES-creER line C2, Th^{tm1(cre/ERT)Nat}, Th^{tm1(cre/Esr1)Nat}
• Gene: Th Location: Chr7:142446516-142453732 bp, - strand Genetic Position: Chr7, 88.06 cM
• Alliance: Th^{tm1(cre/Esr1)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144315
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Th^{tm1(cre/Esr1)Nat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Th (MGI:98735)

• Mutation details: An IRES-creER cassette, a tetracycline resistance gene, and an FRT-flanked neomycin selection marker were all knocked into the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in a sparse number of dopaminergic neurons in the brain. This is line C2. (J:144315)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Th (mouse) Summary of all recombinase alleles driven by Th. Comparative matrix view of recombinase activities Alliance mouse Th gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Th Mutation: 57 strains or lines available

References

• Original: J:144315 Rotolo T, et al., Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology. PLoS One. 2008;3(12):e4099
• All: 16 reference(s)