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Transgene Detail
Symbol: Tg(SLC18A3-cre)Misa
Name: transgene insertion, Hidemi Misawa
MGI ID: MGI:3793863
Synonyms: VAChT-cre
Transgene: Tg(SLC18A3-cre)Misa  Location: unknown  
Strain of Origin:  C57BL/6
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
Mutation detailsThe transgenic construct consisted of an 11.3 kb fragment of the human cholinergic gene locus, containing the first 5 exons of CHAT and the first coding exon of SLC18A3 (located in intron 1 of CHAT). A cassette containing cre followed by IRES-EGFP was inserted at the start codon of the SLC18A3 gene. EGFP expression was undetected. The 11.3 kb fragment included various promoter elements including a distal enhancer, RE1/NRSE, and NGFRE. Founder mice contained approximately 50 copies. Expression of cre recombinase by the human SLC18A3 promoter began at postnatal day 7 and was restricted to a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus. Maximal levels of expression were reached around 8 months ofage, later onset than in the Tg(SLC18a3-cre)KMisa line. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons. (J:86160)
 Tissue activity of this recombinase allele
Driver: SLC18A3     Summary of all recombinase alleles driven by SLC18A3.

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Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:135268 Yamanaka K, et al., Astrocytes as determinants of disease progression in inherited amyotrophic lateral sclerosis. Nat Neurosci. 2008 Mar;11(3):251-3
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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