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Tg(H2-Ea)16Dim
Transgene Detail
Nomenclature
Symbol: Tg(H2-Ea)16Dim
Name: transgene insertion 16, Diane Mathis
MGI ID: MGI:3793709
Synonyms: Ea16, Tg(Ea16)1Strasbourg
Transgene: Tg(H2-Ea)16Dim  Location: unknown  
Transgene
origin
Strain of Origin:  Not Applicable
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(H2-Ea)16Dim expresses 1 gene
 
Mutation detailsAn 8.2 kb construct containing all the mouse (A/J) H2-Ea kappa exons (from clone lambda Charon 4) plus 2 kb of DNA 5' to the cap site and 1.4 kb 3' to the poly(A) addition site was microinjected into (C57BL/6 x SJL) embryos. Two lines 23 and 16 were generated with line 16 carrying 12 copies of the transgene in its genome and line 23 having 4 copies. Northern blots and S1 nuclease analysis showed transcripts in the spleen. Line 16 produced more transcripts than line 4, and both lines showed correct initiation sites of transcription. (J:111128)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Transgenic lines were derived from two transgenic founder mice and designated line 16 and line 23. Line 16 exhibits slightly higher antibody titers and mRNA levels in response to synthetic antigen.
References
Original:  J:111128 Le Meur M, et al., Correcting an immune-response deficiency by creating E alpha gene transgenic mice. Nature. 1985 Jul 4-10;316(6023):38-42
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory