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Targeted Allele Detail
Symbol: En1tm8.1Alj
Name: engrailed 1; targeted mutation 8.1, Alexandra L Joyner
MGI ID: MGI:3789091
Synonyms: En1flox
Gene: En1  Location: Chr1:120602418-120607992 bp, + strand  Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
Germline Transmission:  Earliest citation of germline transmission: J:134516
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring. (J:134516)
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any En1 Mutation:  8 strains or lines available
Original:  J:134516 Sgaier SK, et al., Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development. 2007 Jun;134(12):2325-35
All:  8 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.01
The Jackson Laboratory