Del(MTmt-Tk-mt-Nd5)1Jiha
Spontaneous Allele Detail

Summary

• Symbol: Del(MTmt-Tk-mt-Nd5)1Jiha
• Name: deletion, MT, Jun-Ichi Hayashi 1
• MGI ID: MGI:3776665
• Synonyms: Del1Jiha, deltamtDNA, deltamtDNA4696
• Gene: Del(MTmt-Tk-mt-Nd5)1Jiha Location: unknown Genetic Position: ChrMT, Syntenic

Kyphosis in Polg^tm1Jiha/Polg^tm1Jiha and Del(MTmt-Tk-mt-Nd5)1Jiha mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65025
• Parent Cell Line: TT2F (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Spontaneous (Null/knockout)
• Mutations: Intergenic deletion, Intragenic deletion
• Del(MTmt-Tk-mt-Nd5)1Jiha involves 14 genes/genome features (mt-Tk, mt-Atp8, mt-Atp6 ...) View all
• Mutation details: C2 cells depleted of their mitochondrial DNA were fused with synaptosomal fractions from brain tissue of inbred C57BL/6J mice. One clone possessed a 4696bp deletion that spanned from mt-Tk to mt-Nd5 and encompassed 6 tRNA genes and 7 structural genes. This deletion arose spontaneously and does not have direct repeats surrounding the breakpoint. This allele is maintained within cells that contain 30% mutant mitochondria. (J:65025)

Disease models

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(MTmt-Tk-mt-Nd5)1Jiha Mutation: 0 strains or lines available

Notes

Mice containing this deletion are referred to as mito-mice. Two methods were used to generate mice including transplantation of cytoplasm from enucleated cybrid cells into donor pronuclear stage embryos (J:65025) and fusion of enucleated cybrid cells to mitochondrially depleted TT2F ES cells (J:132407).

References

• Original: J:65025 Inoue K, et al., Generation of mice with mitochondrial dysfunction by introducing mouse mtDNA carrying a deletion into zygotes. Nat Genet. 2000 Oct;26(2):176-81
• All: 14 reference(s)