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Targeted Allele Detail
Symbol: Snord116tm1.1Uta
Name: small nucleolar RNA, C/D box 116 cluster; targeted mutation 1.1, Uta Francke
MGI ID: MGI:3773673
Synonyms: 1-lox, 1-loxp, Snord116del
Gene: Snord116  Location: unknown  Genetic Position: Chr7, Syntenic
Germline Transmission:  Earliest citation of germline transmission: J:131427
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Allele Type:    Targeted (Null/knockout)
Mutation:    Deletion
  Snord116tm1.1Uta involves 9 genes/genome features (Snord116, Snord116l2, Snord116l8 ...) View all
Mutation detailsTwo individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. (J:131427)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Snord116 Mutation:  2 strains or lines available
Original:  J:131427 Ding F, et al., SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice. PLoS One. 2008;3(3):e1709
All:  8 reference(s)

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MGI 6.11
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