Snord116tm1.1Uta
Targeted Allele Detail
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Symbol: |
Snord116tm1.1Uta |
Name: |
small nucleolar RNA, C/D box 116 cluster; targeted mutation 1.1, Uta Francke |
MGI ID: |
MGI:3773673 |
Synonyms: |
1-lox, 1-loxp, Snord116del |
Gene: |
Snord116 Location: unknown Genetic Position: Chr7, Syntenic
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Germline Transmission: |
Earliest citation of germline transmission:
J:131427
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
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Snord116tm1.1Uta involves 9 genes/genome features (Snord116, Snord116l2, Snord116l8 ...)
View all
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Mutation details: Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts.
(J:131427)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:131427 Ding F, et al., SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice. PLoS One. 2008;3(3):e1709 |
All: |
13 reference(s) |
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