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Targeted Allele Detail
Symbol: Pou5f1tm2Jae
Name: POU domain, class 5, transcription factor 1; targeted mutation 2, Rudolf Jaenisch
MGI ID: MGI:3772191
Synonyms: Oct4-EGFP, Oct4-GFP, Oct4-iresGFP, Oct4-IRES-GFP, Pou5f1tm2(EGFP)Jae
Gene: Pou5f1  Location: Chr17:35816929-35821674 bp, + strand  Genetic Position: Chr17, 18.69 cM
Germline Transmission:  Earliest citation of germline transmission: J:130248
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert an "IRES-EGFP-floxed NEO" cassette (containing an internal ribosomal entry site (IRES), enhanced green fluorescent protein (EGFP) sequence, and a loxP-flanked neo) between the stop codon and endogenous polyA signal of the targeted gene. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the neo selection cassette. (J:130248)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 4 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:  79 strains or lines available
When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-EGFP murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.
Original:  J:130248 Lengner CJ, et al., Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell. 2007 Oct 11;1(4):403-415
All:  30 reference(s)

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MGI 6.17
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