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Dnmt1tm1Okan
Targeted Allele Detail
Summary
Symbol: Dnmt1tm1Okan
Name: DNA methyltransferase (cytosine-5) 1; targeted mutation 1, Masaki Okano
MGI ID: MGI:3769043
Synonyms: Dnmt1ps
Gene: Dnmt1  Location: Chr9:20818505-20871184 bp, - strand  Genetic Position: Chr9, 7.66 cM
Alliance: Dnmt1tm1Okan page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:129049
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Nucleotide substitutions
 
Mutation detailsA point mutation in exon 32 introduced an amino acid substituation of serine for cysteine at position 1229 (C1229S). This mutation results in an abrogation of catalytic activity. Additionally, an IRES-betageo cassette was inserted into an intron upstream of the point mutation and was subsequently removed by cre-mediated recombination. Expression of the full-length protein was confirmed by western blot analysis on embryonic stem cell extracts. (J:129049)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dnmt1 Mutation:  88 strains or lines available
References
Original:  J:129049 Takebayashi S, et al., Major and essential role for the DNA methylation mark in mouse embryogenesis and stable association of DNMT1 with newly replicated regions. Mol Cell Biol. 2007 Dec;27(23):8243-58
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/22/2022
MGI 6.22
The Jackson Laboratory