Pgr^{tm3(rtTA2S*M2)Lyd}
Targeted Allele Detail

Summary

• Symbol: Pgr^{tm3(rtTA2S*M2)Lyd}
• Name: progesterone receptor; targeted mutation 3, John P Lydon
• MGI ID: MGI:3768562
• Synonyms: PR^rtTA
• Gene: Pgr Location: Chr9:8899834-8968612 bp, + strand Genetic Position: Chr9, 2.46 cM
• Alliance: Pgr^{tm3(rtTA2S*M2)Lyd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128735
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: An improved reverse tetracyclin-dependent transactivator (rtTA2^S-M2) and a floxed neo cassette were inserted into exon 1. Expression of the transactivator is under the control of the endogenous promoter. Northern blot analysis was performed to confirmed expression of rtTA in mammary gland and uterus. The insertion also results in a null allele as stated by the authors. (J:128735)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pgr Mutation: 73 strains or lines available

References

• Original: J:128735 Mukherjee A, et al., Targeting reverse tetracycline-dependent transactivator to murine mammary epithelial cells that express the progesterone receptor. Genesis. 2007 Oct;45(10):639-46
• All: 1 reference(s)