Tg(APOE_i3*,APOC1)2Lmh
Transgene Detail

Summary

• Symbol: Tg(APOE_i3*,APOC1)2Lmh
• Name: transgene insertion 2, Louis M Havekes
• MGI ID: MGI:3768068
• Synonyms: apoE3-Leiden, APO*E3-Leiden, APOE*3Leiden, APOE*3-Leiden, APOE*3Leiden-C1, E3L, Tg(APOE*3,APOC1)2Lmh
• Transgene: Tg(APOE_i3*,APOC1)2Lmh Location: unknown
• Alliance: Tg(APOE_i3*,APOC1)2Lmh page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(APOE_i3*,APOC1)2Lmh expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOC1 (341)

  Apoc1

  (MGI:88053)

  human

  APOE (348)

  Apoe	(MGI:88057)

• Tg(APOE_i3*,APOC1)2Lmh expression driven by 2 genes
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  APOC1 (341)

  Apoc1

  (MGI:88053)

  human

  APOE (348)

  Apoe	(MGI:88057)

• Mutation details: A construct carrying a 27-kb genomic fragment containing the human APOE*3-Leiden gene and endogenous promoter, the APOCl gene, and the complete intergenic region between APOCl and the APOCl pseudogene, harboring the postulated liver regulatory element, was used to create transgenic mice. The human APOE*3-Leiden mutation is characterized by a tandem duplication of codons 120-126 and yields a mature protein of 306 amino acid residues. This transgenic line carries approximately 4 copies of the transgene. Expression of APOE*3 and APOC1 mRNA and protein is detectable in liver of transgenic mice and not in kidney. Two other transgenic lines, 181 and 195, were also generated. (J:109205)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:109205 van den Maagdenberg AM, et al., Transgenic mice carrying the apolipoprotein E3-Leiden gene exhibit hyperlipoproteinemia. J Biol Chem. 1993 May 15;268(14):10540-5
• All: 91 reference(s)