Tg(Cd3d-Il5)NJ.1638Nal
Transgene Detail

Summary

• Symbol: Tg(Cd3d-Il5)NJ.1638Nal
• Name: transgene insertion NJ.1638, Nancy A Lee
• MGI ID: MGI:3767981
• Synonyms: IL-5tg, NJ.1638
• Transgene: Tg(Cd3d-Il5)NJ.1638Nal Location: unknown
• Alliance: Tg(Cd3d-Il5)NJ.1638Nal page

Transgene origin

• Strain of Origin: (CBA/J x C57BL/6J)F1 x C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cd3d-Il5)NJ.1638Nal expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Il5 (MGI:96557)

• Mutation details: This construct was created by fusing in frame a 170-bp fragment from the 5' end of IL-5 cDNA with the remaining portion of the genomic IL-5 gene. This minigene has no upstream flanking sequences but has all of the introns of the gene as well as 1 .2 kb of downstream flanking sequence. To drive expression of the transgene in T cells, a promoter and tissue-specific enhancer derived from the murine CD3d gene was included upstream. Three founding lines (NJ.759, NJ.1638, NJ.1643) were created with one chosen for further studies. High levels of circulating IL-5 were detected in the sera of transgenic mice but not in wild-type mice. (J:123440)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:123440 Lee NA, et al., Expression of IL-5 in thymocytes/T cells leads to the development of a massive eosinophilia, extramedullary eosinophilopoiesis, and unique histopathologies. J Immunol. 1997 Feb 1;158(3):1332-44
• All: 57 reference(s)