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Transgene Detail
Symbol: Tg(Tek-Jam3)1Maal
Name: transgene insertion 1, Michel Aurrand-Lions
MGI ID: MGI:3767832
Synonyms: pHHNS-JAM-C, Tg(Tie2-Jam-C)1Maal
Transgene: Tg(Tek-Jam3)1Maal  Location: unknown  
Strain of Origin:  CBA x C57BL/6
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Tek-Jam3)1Maal expresses 1 gene
Mutation detailsThe transgene contains the coding sequence, 3' untranslated region and polyadenylation signal of mouse junction adhesion molecule 3 (Jam3) inserted near the 3' end of the first exon of the mouse endothelial-specific receptor tyrosine kinase gene (Tek) in a genomic DNA fragment containing 2.1 kb of upstream DNA containing Tek promoter sequences, most of exon 1 and, following the inserted Jam3 sequence, ~10 kb of the first half of intron 1, which contains endothelial cell-specific enhancer elements.

Western blot analysis of total lung lysates and of immunoprecipitates of lung lysates demonstrate elevated expression of JAM3 in lungs of transgenic mice versus control littermates. Flow cytometric analysis of dissociated lung tissue cells stained for JAM3, the endothelial-cell marker VE-cadherin and the hematopoietic cell marker CD45 show 3-fold enhanced expression of JAM3 specifically on endothelial cells. (J:99020)

View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 3 anatomical structures
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:99020 Aurrand-Lions M, et al., Junctional adhesion molecule-C regulates the early influx of leukocytes into tissues during inflammation. J Immunol. 2005 May 15;174(10):6406-15
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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