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Trpc1tm1Lbi
Targeted Allele Detail
Nomenclature
Symbol: Trpc1tm1Lbi
Name: transient receptor potential cation channel, subfamily C, member 1; targeted mutation 1, Lutz Birnbaumer
MGI ID: MGI:3764882
Gene: Trpc1  Location: Chr9:95587135-95632428 bp, - strand  Genetic Position: Chr9, 50.2 cM, cytoband E4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:127788
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsAn EcoRI site in exon 8 was converted to a stop codon and a PGK-neo selection cassette replaced the terminal part of exon 8 along with intron 8. This resulted in loss of the coding region for amino acids 584 - 670 encoding transmembrane segments 4 and 5 and a small part of the putative pore domain. RT-PCR analysis detected a shorter transcript in which all of exon 8 was skipped resulting in a frameshift and stop codon at position 2 of exon 9. Western blot analysis confirmed absence of expression in the submandibular glands of homozygous mice. (J:127118, J:127788)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trpc1 Mutation:  26 strains or lines available
References
Original:  J:127788 Dietrich A, et al., Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1. Pflugers Arch. 2007 Dec;455(3):465-77
All:  44 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory