Lgr5^{tm1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm1(cre/ERT2)Cle}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1, Hans Clevers
• MGI ID: MGI:3764660
• Synonyms: Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5^{tm1(cre/ESR1)Cle}, Lgr5^UT
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127123
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Lgr5^{tm1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr5 (MGI:1341817)

• Mutation details: An EGFP-IRES-creERT2 cassette was inserted into exon 1 of the gene along with a loxP-flanked neomycin cassette. The neomycin gene was then excised in vivo by crossing with cre-deleter strain. GFP expression is driven by the endogenous promoter. Cre expression is induced in Lgr5 expressing cells by tamoxifen treatment. (J:127123)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

Expression

In Mice Carrying this Mutation:	40 assay results 6 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Lgr5^{tm1(cre/ERT2)Cle}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 57 strains or lines available

References

• Original: J:127123 Barker N, et al., Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007 Oct 25;449(7165):1003-7
• All: 532 reference(s)