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Fn1tm4Hyn
Targeted Allele Detail
Nomenclature
Symbol: Fn1tm4Hyn
Name: fibronectin 1; targeted mutation 4, Richard Hynes
MGI ID: MGI:3764511
Synonyms: EIIIA-EIIIB-, EIIIA/EIIIB-double-null
Gene: Fn1  Location: Chr1:71585520-71653200 bp, - strand  Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:126737
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsES cells from homozygous Fn1tm1Ksek male mouse in which exon EDB (EIIIB) was deleted and replaced with a singe loxp site was isolated and targeted with the same construct used in Fn1tm1Bwg. Following homologous recombination and transient cre-mediated recombination, a single loxp site was left in place of the exon encoding the EIIIA domain. Northern and Western blot confirmed the absence of EIII1 and EIIIB mRNA and protein and that deletion of both of those exons did not compromise the expression of FN mRNA and protein, nor the deposition of FN into the matrix. (J:126737)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Fn1 Mutation:  60 strains or lines available
Notes
ES cells were derived from male homozygous Fn1tm1Ksek mouse of a mixed 129P2/OlaHsd and C57BL/6J background strain.
References
Original:  J:126737 Astrof S, et al., Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin. Dev Biol. 2007 Nov 1;311(1):11-24
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory